IPI’s Control Antibody Collection supports assay validation by helping to assess background signals, identify non-specific binding and improve confidence in results. Used as regular controls in the IPI lab, these proteins are designed to match the host species and isotype of our primary antibodies. They’re optimized for compatibility across a range of common applications, including flow cytometry, immunofluorescence, immunohistochemistry and ELISA.
IPI-MOPC21 is compatible with all IPI rabbit IgG isotype antibodies and regularly used as a negative control in all IPI applications. IPI’s anti-V5 rabbit IgG antibody recognizes the V5 epitope on tagged proteins, and commonly serves as an expression and detection control across the IPI lab.
All IPI antibodies go through extensive characterization, including:
- Molar mass verification via mass spectrometry
- Purity and structural integrity analysis via size exclusion chromatography
- Affinity and kinetics measurements via surface plasmon resonance
- Non-specific binding assessment via polyspecificity reagent assay testing
- Activity analysis via flow cytometry
ID | Product name | Description | Isotype/ Format | Specificity | Applications | |
|---|---|---|---|---|---|---|
218107 | Anti-V5 [IPI-SV5-Pk1] | Anti-V5 tag chimeric recombinant antibody with fused human variable and… more+ Anti-V5 tag chimeric recombinant antibody with fused human variable and rabbit constant domains; binds to GKPIPNPLLGLDST sequence. less- | Rabbit IgG | V5 tag (GKPIPNPLLGLDST) | Flow Cytometry, Western Blot | |
237822 | Rabbit IgG Isotype Control [IPI-MOPC-21] | Anti-Rabbit IgG Isotype Control [IPI-MOPC-21] (human) chimeric recombinant antibody with… more+ Anti-Rabbit IgG Isotype Control [IPI-MOPC-21] (human) chimeric recombinant antibody with fused human variable and rabbit constant domains. less- | Rabbit IgG | Unknown specificity - acts as isotype (negative) control | Flow , IF |
For neurons to communicate, their axons must navigate precisely to their targets through a complex…
Neuronal communication and neurological function rely on chemical signaling across a narrow extracellular space known…
Epitope tags are short amino acid sequences fused to a protein’s N- or C-terminus. When…
Need information or assistance with one of our antibodies?
Go to the Addgene Help Center or contact Addgene below.