Epitope tag antibody collection

About the collection

Epitope tags are short amino acid sequences fused to a protein’s N- or C-terminus. When paired with an antibody that specifically recognizes this sequence, epitope tags can help purify, detect or localize particular proteins. However, epitope tag antibodies can be expensive and, therefore, difficult to obtain.

To fill the gap, IPI developed recombinant versions of antibodies that recognize common epitope tags, including V5, Protein C, DYKDDDDK, Rho, His, Biotin, EE, GCN4 and Strep. These antibodies are now easily and inexpensively available from IPI through Addgene.

All IPI antibodies go through extensive characterization, including:

Molar mass verification via mass spectrometry
Purity and structural integrity analysis via size exclusion chromatography
Affinity and kinetics measurements via surface plasmon resonance
Non-specific binding assessment via polyspecificity reagent assay testing
Activity analysis via flow cytometry

True to our open science model, we are sharing the antibody sequences with purchasers and releasing the plasmids that encode the antibodies.

This is our first set of tool antibodies. Our goal is to make them both renewable and broadly available.

Rob Meijers
Head of the IPI Neuroscience Program

Recombinant antibody list

ID	Product name	Description	Source species	Isotype	Specificity	Applications
218102	Anti-Biotin [IPI-M33]	Anti-Biotin chimeric recombinant antibody with fused human variable and rabbit… more+ Anti-Biotin chimeric recombinant antibody with fused human variable and rabbit constant domains; binds to biotin. less-	Rabbit	IgG1	Biotin	Flow Cytometry, Western Blot	Purchase with Addgene
218103	Anti-EE [IPI-3D5EE_48.K]	Anti-EE tag chimeric recombinant antibody with fused human variable and… more+ Anti-EE tag chimeric recombinant antibody with fused human variable and rabbit constant domains; binds to EYMPME sequence. less-	Rabbit	IgG1	EE tag (EYMPME)	Flow Cytometry, Western Blot	Purchase with Addgene
218104	Anti-GNC4 [IPI-C11L34]	Anti-GCN4 tag chimeric recombinant antibody with fused human variable and… more+ Anti-GCN4 tag chimeric recombinant antibody with fused human variable and rabbit constant domains; binds to HLENEVARLKK sequence. less-	Rabbit	IgG1	GCN4 (HLENEVARLKK)	Flow Cytometry, Western Blot	Purchase with Addgene
218105	Anti-Strep [IPI-C23.21]	Anti-Strep tag chimeric recombinant antibody, with fused human variable and… more+ Anti-Strep tag chimeric recombinant antibody, with fused human variable and rabbit constant domains; binds to WSHPQFEK sequence. less-	Rabbit	IgG1	Strep tag (WSHPQFEK)	Flow Cytometry, Western Blot	Purchase with Addgene
218106	Anti-DYKDDDDK [IPI-4E11/M1]	Anti-DYKDDDDK (Flag) tag chimeric recombinant antibody with fused human variable… more+ Anti-DYKDDDDK (Flag) tag chimeric recombinant antibody with fused human variable and rabbit constant domains; binds in a calcium-dependent manner to the N-terminal DYKDDDDK sequence. less-	Rabbit	IgG1	N-terminal DYKDDDDK tag	Flow Cytometry, Western Blot	Purchase with Addgene
218107	Anti-V5 [IPI-SV5-Pk1]	Anti-V5 tag chimeric recombinant antibody with fused human variable and… more+ Anti-V5 tag chimeric recombinant antibody with fused human variable and rabbit constant domains; binds to GKPIPNPLLGLDST sequence. less-	Rabbit	IgG1	V5 tag (GKPIPNPLLGLDST)	Flow Cytometry, Western Blot	Purchase with Addgene
218108	Anti-ProC [IPI-HPC-4]	Anti-Protein C tag chimeric recombinant antibody with fused human variable… more+ Anti-Protein C tag chimeric recombinant antibody with fused human variable and rabbit constant domains; binds to EDQVDPRLIDGK sequence. less-	Rabbit	IgG1	ProC tag (EDQVDPRLIDGK)	Flow Cytometry, Western Blot	Purchase with Addgene
218109	Anti-His [IPI-HAP1/5.13.22]	Anti-His tag chimeric recombinant antibody with fused human variable and… more+ Anti-His tag chimeric recombinant antibody with fused human variable and rabbit constant domains; binds selectively to the C-terminal HHHHHHHHHH sequence. less-	Rabbit	IgG1	C-terminal HHHHHH tag	Flow Cytometry, Western Blot	Purchase with Addgene
218110	Anti-Rho [IPI-1D4]	Anti-Rhodopsin chimeric recombinant antibody with fused human variable and rabbit… more+ Anti-Rhodopsin chimeric recombinant antibody with fused human variable and rabbit constant domains; binds to TETSQVAPA sequence. less-	Rabbit	IgG1	Rho tag (TETSQVAPA)	Flow Cytometry, Western Blot	Purchase with Addgene

IPI plasmids

Epitope tag antibody plasmids

In addition to our epitope tag antibodies, IPI offers the plasmids that encode these antibodies. These vectors (dubbed “pTipi,” for plasmid transient expression at IPI) help researchers overcome cost and scale challenges with minimal intellectual property concerns.

Plasmid scaffold

IPI also shares our pTipi plasmid scaffold, the plasmid used to create our epitope tag antibodies, for researchers to produce other proteins of interest.

View IPI plasmids

Looking for additional protein resources?

Reach out to our team at [email protected].

Technical support

Need information or assistance with one of our antibodies?

Go to the Addgene Help Center or contact Addgene below.

Contact Addgene