Many proteins are evolutionarily conserved, rendering the production of monoclonal antibodies through animal immunization challenging, if not impossible. We have developed a solution: a technology based on yeast display that mimics the variability of human antibody responses.
Yeast transformed with one of three different light chains, tagged with c-Myc.
Yeast with light chain transformed with one of five different heavy chains, tagged with V5. Variable region (CDRH3) is transformed independently alongside a helper plasmid.
Yeast express light and heavy chains, which anchor on the cell surface via two yeast proteins. This creates a unique antigen binding fragment (Fab) that recognizes a specific region of an antigen.