Our platform combines wide-ranging protein science expertise with innovative systems and technologies to amplify the power of protein tools. We leverage the platform in multiple ways, exploring collaborations with bioscientists around the world and developing high-quality synthetic recombinant antibodies and critical protein tools for the research community.
Antigen production
Our team understands the importance of getting to know each of our target proteins. We study the structure and function of these targets, and determine the specific biological questions antibodies may help address. Then, we craft optimal antigen constructs and rigorously screen them to ensure they remain soluble, fold correctly and do not aggregate.
Antibody discovery
We use in vitro selection from synthetic libraries to discover antibodies with precise binding properties. Our libraries, containing up to 10^10 unique sequences displayed on yeast or bacteriophage, allow us to tailor selection strategies for specific traits like high affinity, off-target selectivity or unique functions such as ligand blocking. By thoroughly sampling, sequencing, and analyzing binders and non-binders, we uncover patterns that reveal the key factors for successful antibody development.
Antibody production & characterization
We use high-throughput methods and state-of-the-art automation to transfect, express, harvest and purify our candidate antibodies, basing them on mammalian cell lines suitable for research, diagnostic and therapeutic uses. We then characterize our antibodies to confirm their integrity, activity and functionality, and validate them in multiple assays. Once we’re sure of an antibody’s performance and applicability, we scale up production and share our reagents with researchers worldwide.
Meeting the IPI standard
Reagent antibodies are valuable, but notorious for issues of performance and reproducibility. To address this, our antibodies are extensively characterized and validated. We disclose this data — along with our antibody sequences, reagent concentrations, and more — to those who use our reagents because secrets have no place in science.
Application testing
We test our antibodies in several common applications and are constantly expanding our lab capabilities to develop more specialized assays. At the same time, we’re well aware that antibody performance can vary in different labs and different research questions necessitate different applications. To build a comprehensive picture of each antibody, we work closely with an international community of antibody users who volunteer to test our beta-stage antibodies and report back on real-world assay performance.
